Ye Z.*, Shelton A.M.*, Shaker J.R., Boussard J., Colonell J., Birman D., Manavi S., Chen S., Windolf C., Hurwitz C., Namima T., Pedraja F., Weiss S., Raducanu B., Ness T.V., Jia X., Mastroberardino G., Rossi L.F., Carandini M., Häusser M., Einevoll G.T., Laurent G., Sawtell N.B., Bair W., Pasupathy A., Lopez C.M., Dutta B., Paninski L., Siegle J.H., Koch, C., Olsen S.R., Harris T.D., Steinmetz N.A. Ultra-high density electrodes improve detection, yield, and cell type identification in neuronal recordings, bioRxiv, (2024).
We contributed to the developement of a silicon probe which samples neuronal activity at ultra-high spatial density (~10 times higher than previous probes), called Neuropixels Ultra. We demonstrate that NP Ultra can record signals from cortical neuronal dendrites with high SNR.
Rossi, L.F. †, Harris, K.D., Carandini M. Spatial connectivity matches direction selectivity in visual cortex, Nature, (2020).
We combined genetics, rabies virus tracing and 2 photon imaging, to provide the first anatomical and functional characterization of a neuron’s excitatory and inhibitory presynaptic ensemble. We discovered spatial patterns of excitatory and inhibitory connectivity generating orientation and direction tuning in V1.
Rossi, L.F.†, Wykes†, R.C., Kullmann, D.M., Carandini, M. Focal cortical seizures start as standing waves and propagate respecting homotopic connectivity. Nature communications, (2017).
We investigated the spatio-temporal dynamics of focal seizures with widefield calcium imaging, electrophysiology and pharmacology in mouse visual cortex. We demonstrate seizures start as local synchronous activations and propagate along the connectivity that underlies normal sensory processing.
Rossi, L.F. †, Kullmann, D.M., Wykes†, R.C. The Enlightened Brain: Novel Imaging Methods Focus on Epileptic Networks at Multiple Scales. Frontiers in Cellular Neuroscience, (2018).
A perspective on how novel imaging methods at multiple scales can elucidate the mechanisms underlying the development of epilepsy and the generation of seizures.
Pachitariu, M., Stringer, C., Schröder, S., Dipoppa, M., Rossi, L.F., Dalgleish, H., Carandini, M., and Harris, K.D. Suite2p: beyond 10,000 neurons with standard two-photon microscopy, Preprint, bioRxiv, (2017).
We introduced innovative methods for two-photon data analysis (i.e. image registration, segmentation, spike deconvolution). We implemented them in open-source software which has been widely adopted by the neuroscience community, providing a major step forward in neuronal recordings.
Ganmor, E., Krumin, M., Rossi, L.F., Carandini, M., and Simoncelli E.P. Direct estimation of firing rates from calcium imaging data, Preprint, arXiv, (2016).
Calcium imaging indirectly reports neuronal firing. We developed a generative model to estimate rates, spike count histograms, or tuning curves directly from calcium indicators measurements.
Carandini, M., Shimaoka, D., Rossi, L.F., Sato, T.K., Benucci, A., Knoepfel, T. Imaging the Awake Visual Cortex with a Genetically Encoded Voltage Indicator. Journal of Neuroscience, (2015).
We pioneered simultaneous ratiometric wide-field imaging of neuronal activity and haemodynamic responses in awake mice by means of genetically encoded voltage sensitive fluorescence proteins (VSFP-Butterfly 2.1) ans genetically encoded calcium indicators (GCaMP3).
Brondi, M.*, Sato, S.S.*, Rossi, L.F., Ferrara, S., Ratto, G.M. Finding a needle in a haystack: identification of EGFP tagged neurons during calcium imaging by means of two-photon spectral separation. Frontiers in Molecular Neuroscience, (2012).
Two-photon imaging is limited by the palette of indicators. We demonstrated that multi-spectral two-photon imaging can un-mix fluorophores with overlapping emission but different excitation spectra in vivo
*contributed equally
†corresponding author