The lab combines methods to bridge function, connectivity, and gene expression in neural circuits in vivo, using the mouse as a model system. Our toolkit includes:
Large-scale two-photon calcium imaging
We record simultaneously the activity of thousands of individual neurons, across layers or cortical areas, using large-scale, volumetric two-photon calcium imaging.
Trans-synaptic connectivity tracing
We are one of the few labs that can visualise, in vivo, the ensemble of synaptic patterns individual neurons of choice using trans-synaptic viral tracers.
Single cell electroporation
We introduce new DNA and modify gene expression in single neurons in vivo with single-cell electroporation.
Dendritic glutamate and calcium imaging
We simultaneously image presynaptic neurotransmitter release and post-synaptic calcium responses on single dendrites.
Photoablation and dendritic pruning
We selectively eliminate individual neurons or prune single dendrites with laser ablation.
We relate functional and anatomical neuronal diversity to gene expression by profiling neurons recorded in vivo with spatial transcriptomics.
Wide-field calcium and blood-flow imaging
We map brain areas and measure mesoscale patterns of activity with wide-field imaging of activity reporters or blood flow.
High-density electrophysiology and optogenetics
We use high-density micro-electrode arrays (e.g. Neuropixels probes) to record spikes at neuronal somas and dendrites with high temporal precision in vivo.
Virtual reality and naturalistic behaviours
Keep an eye out! We are developing new behavioural paradigms to study how animal’s adaptively pursuit visual targets and stabilise visual perception during voluntary movements.